Traditional methods of oxidizing alcohols often rely on toxic reagents, as well as high pressures and temperatures, which can be hazardous. In contrast, biocatalysis utilizes enzymes as natural and non-toxic catalysts, typically under milder reaction conditions. This study aims to perform oxidation reactions of primary and secondary alcohols using enzymes as catalysts. Specifically, we will employ the alcohol dehydrogenase enzyme from Sphingobium yanoikuyae (SyADH) and a laccase derived from Trametes versicolor to facilitate these oxidation processes in an environmentally friendly manner, eliminating the need for extreme temperatures and pressures.The reactions involving laccase are carried out in a biphasic medium using MTBE as a solvent. However, we are actively researching ways to improve this system. When the reaction was conducted using a buffer solution, side products were generated, which did not align with the principles of green chemistry. Even though, we can achieve conversions of alcohols to their corresponding carbonyl compounds, thus promoting a greener and more sustainable approach to these oxidation reactions.