Chitin, the most abundant aminopolysaccharide, can be found in the exoskeleton of crustacean shells, a major waste product of the fishing industry. The water-soluble derivative of chitin, chitosan, acquired through a process of deacetylation affords derivatization and processability of this natural biopolymer. Traditionally, deacetylation processes utilizing harsh chemical conditions and high energy inputs limiting scalability and sustainable practices. Furthermore, these methods result in depolymerization, presenting low molecular weight fragments. The combination of mechanochemistry and aging has proven to yield high molecular weight chitosan while using low energy and solvent quantities however requiring prolonged reaction times. Resonance Acoustic Mixing (RAM) leverages high-frequency and low-amplitude mechanical energy to achieve rapid, efficient, and uniform mixing, reduced chemical usage and reaction time, as well as improved yield. In this study, RAM is investigated as a viable alternative to effectively and sustainably deacetylate chitin. Comparative analysis of this method with traditional processes will be performed to evaluate the efficacy of RAM in achieving high degrees of deacetylation. The manipulation of parameters such as time, temperature, and reaction conditions will systematically be optimized to yield high molecular weight fragments. This research aims to further the advancement of green processing methods for biopolymer modification, contributing to the sustainable production of high-performance biomaterials. Improving the sustainability of chitosan production by optimizing energy usage through RAM reducing the overall environmental footprint. Additionally, the potential high molecular weight fragments isolated by RAM can be functionalized and used to produce sustainable materials, specifically materials used within water treatment facilities.