The use of marine-derived biomaterials in tissue engineering presents an exciting opportunity to create sustainable, regenerative solutions. This research focuses on the innovative extraction of collagen from Corvina fish skin, a by-product of the fishing industry, and its combination with chitosan derived from the shells of marine invertebrates. By utilizing these abundant, often discarded resources, we are not only advancing tissue engineering but also embracing the principles of green chemistry and promoting a circular economy. Green chemistry is at the heart of this work, as it relies on the transformation of fishing and seafood industry waste—materials that would otherwise be discarded—into valuable, functional biomaterials. Extracting collagen from Corvina fish skin and chitosan from invertebrate shells reduces the need for synthetic, petroleum-based alternatives, making the process more environmentally sustainable. These biomaterials are biodegradable, non-toxic, and capable of providing the structural support needed for tissue regeneration, offering an eco-friendly solution that aligns with the principles of green chemistry. Moreover, the use of fish skin and invertebrate shells contributes to a circular economy, where waste is repurposed to create high-value products, reducing environmental impact while offering economically viable solutions for the biotechnology sector. This approach not only opens new possibilities for tissue engineering but also creates an opportunity for the fishing industry to tap into an additional revenue stream, further enhancing the economic sustainability of the project. In the future, optimizing extraction methods to minimize energy consumption and waste production could improve the sustainability of this process even further, ensuring that the development of regenerative medicine remains both innovative and environmentally responsible.