Bioguided Fractionation of Modified Plant Extract: An Efficient Approach for Discovering Bioactive Compounds and Identifying Biological Targets
This study presents a methodology for bioactivity-guided fractionation of chemically modified Ambrosia tenuifolia extracts using mild derivatization conditions at room temperature. New trypanocidal compounds less toxic than the starting materials were obtained in a single reaction step. In addition, bioinformatics tools were used to identify potential biological targets for Trypanosoma cruzi, reducing the need for extensive biological testing.