Use of lignocellulosic biomass in continuous processes in biorefineries poses challenges due to its recalcitrant properties, feedstock variability, and materials handling of solids at large scale. Limitations include lignin derived inhibitors, and resistance to mixing due to rheological properties of lignocellulosic particulates at high solids loadings. Thus, this research examines formation of high loading slurries of corn stover at solid loadings of 300 g/L, and explores the role of low levels of cellulase enzymes on liquefaction in a fed batch process using the commercial enzymes Celluclast 1.5L or Ctec-2 at 1FPU/g or 3 FPU/g of dry solids, basis. Corn stover pellets were added into a 1 L stirred bioreactor containing enzyme solution in a fed-batch manner over a 5-hour period until reaching 30% solids loading (dry wgt / vol basis). After 6, 24 and 96 hours, samples were taken and characterized with respect to their sugar composition, rheology, water absorption, free water and enzyme activity. Slurries with dramatically reduced yield stresses were obtained compared to controls prepared without enzyme. Yield stresses of 178±7 Pa (3 FPU, Celluclast 1.5L) and 79±6 Pa (3 FPU, Ctec-2) were measured for corn stover at 6 and 24 hours, compared to 6,000 Pa for samples without enzyme. Yield stress was 155± 29 Pa (3FPU, Ctec-2) and 257 ± 72 Pa (1 FPU, Celluclast 1.5L) for corn cobs at 24 hours. Enzyme liquefaction and its ability to form high concentration slurries before pretreatment holds the potential to develop new and scalable processing strategies for cellulose biorefineries.