Lactones, salts, crystals, and other forms of high-purity glucaric acid through fermentation using microbes from biomass. kalion Inc. uses patented microbes from biomass through synthetic biological processes to produce lactones, salts, crystals, and other forms of high-purity glucaric acid through fermentation. Glucaric acid can replace phosphates and chelating agents in detergents and waste treatment. The traditional use of phosphates and chelating agents may include eutrophication, which may interfere with the removal and treatment of toxic metals. The traditional production of glucaric acid is through the nitric-acid oxidation of glucose. This new biological process produces higher-purity glucaric acid with cheaper and safer materials and less waste.