Technology that uses a protein tag that selectively binds to amino acids to measure protein concentration. The Sprint® Rapid Protein Analyzer uses iTAG® protein-tagging technology. The iTAG® solution selectively binds to three amino acids present in proteins: histidine, arginine, and lysine. The iTAG® solution is acidic and connects with the basic group of these amino acids when they come into contact. After removing the iTAG® solution through filtration, colorimetry is used to determine protein concentration. Because the iTAG solution is selective, it does not interact with other nitrogenated compounds, making it easier to identify adulterated products. This process is more efficient than traditional methods, taking less than 5 minutes (traditional Kjeldahl testing takes 4 hours). The iTAG® solution is also unreactive, soluble in water, and harmless. The overall process is also cheaper than traditional methods and produces no waste. Traditional protein testing (Kjeldahl and combustion testing) requires high energy inputs, hazardous chemicals reagents, and metal catalysts.
EPA Presidential Green Chemistry Challenge: 2009 Greener Reaction Conditions Award
