Effect of inorganic carbon sources in succinic acid biosynthesis using acid-tolerant engineered yeast
The biological process of succinic acid (SA) production can simultaneously reduce the dependency on fossil-based resources and sequester CO2 to synthesize value-added products. In the present study, an acid-tolerant engineered strain was employed to minimize waste (e.g., gypsum) generation in downstream processing and contamination risks. The cost of the fermentation medium is a crucial factor for the industrial production of SA. Corn steep liquor (CSL) was utilized to formulate a low-cost medium for synthesizing SA.