The biological process of succinic acid (SA) production can simultaneously reduce the dependency on fossil-based resources and sequester CO2 to synthesize value-added products. In the present study, an acid-tolerant engineered strain was employed to minimize waste (e.g., gypsum) generation in downstream processing and contamination risks. The cost of the fermentation medium is a crucial factor for the industrial production of SA. Corn steep liquor (CSL) was utilized to formulate a low-cost medium for synthesizing SA. Inorganic carbon plays a pivotal role in the reductive TCA cycle in the SA pathway. Therefore, different inorganic carbon sources and their synergistic effects were studied in the CSL-based medium. Preliminary studies in shake flasks showed a positive impact on SA titer. Furthermore, the synergistic effect of CO2 and carbonic acid resulted in a maximum SA titer of 35 g/L in the bench-top fermenters. However, it did not significantly influence biomass growth. This result will help in designing end-to-end sustainable bio-manufacturing processes at an industrially relevant scale with the help of life cycle assessment and techno-economic analysis tools.