Three-Steps, One-Pot Synthesis for dNTPs in Polymerase Chain Reactions

Manufacturing of polymerase chain reaction (PCR) reagents. PCR is used in research, genetic engineering, forensics, infectious disease identification, food safety, and personalized medicine. The conventional production of key chemicals for PCR tests (such as deoxyribonucleotide triphosphates, or dNTP) is hazardous, inefficient, and not atom economic. This new method for the manufacturing of dNTP consists of only three steps in a single pot, eliminating hazardous reagents and solvents such as zinc chloride, triphenyl phosphine, aldrithiol, dimethyl formamide, and dichloromethane. Based on empirical data, this process reduces organic solvent consumption by up to 95% and hazardous waste production by up to 65%.