Infections associated with medical devices are a serious global issue, affecting millions of patients and increasing healthcare costs . These infections are primarily due to the formation of biofilms on the surfaces of devices such as catheters and implants, which act as protective barriers against antimicrobial treatments and compromise device functionality. Additionally, infections in indwelling devices contribute to environmental pollution due to the frequent need for replacements, further increasing the burden on healthcare system. This study presents an antimicrobial and biocompatible coating made from amyloid aggregates of bovine serum albumin (BSA) on glass and PVC (Polyvinyl Chloride), designed to inhibit the adhesion of Staphylococcus aureus (S.A), a common pathogen in medical device infections. The addition of vancomycin on adhered bacteria on the coating enhances the antimicrobial effect, offering a sustainable solution rooted in green chemistry principles. BSA, as a natural and renewable protein, reduces reliance on synthetic materials, aligning this approach with environmentally friendly and sustainable practices